125000003729 nucleotide group Chemical group 0.000 description 52.
238000003752 polymerase chain reaction Methods 0.000 description 56. 238000004519 manufacturing process Methods 0.000 claims description 5. 229920001405 Coding region Polymers 0.000 claims description 22. 230000035897 transcription Effects 0.000 claims description 26. 108090000623 proteins and genes Proteins 0.000 claims description 39. 102000004169 proteins and genes Human genes 0.000 claims description 39. 102000004965 antibodies Human genes 0.000 claims description 82. 229920003013 deoxyribonucleic acid Polymers 0.000 claims description 108. 229920001850 Nucleic acid sequence Polymers 0.000 claims description 121. 238000010276 construction Methods 0.000 claims abstract description 17. 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 43. 230000003321 amplification Effects 0.000 claims abstract description 43. 108020004707 nucleic acids Proteins 0.000 claims abstract description 371. 150000007523 nucleic acids Chemical class 0.000 claims abstract description 578. 230000002068 genetic Effects 0.000 title claims abstract description 68. Assignors: WOODRUFF, Lauren Status Pending legal-status Critical Current Links ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: MIKKELSEN, Tarjei Assigned to THE BROAD INSTITUTE, INC. Assignors: NICOL, ROBERT Assigned to THE BROAD INSTITUTE, INC. Assignors: VOIGT, CHRISTOPHER Assigned to THE BROAD INSTITUTE, INC. Assignors: NICOL, ROBERT Assigned to MASSACHUSETTS INSTITUTE OF TECHNOLOGY reassignment MASSACHUSETTS INSTITUTE OF TECHNOLOGY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). 
Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority to US201462003331P priority Critical Application filed by Massachusetts Institute of Technology, Broad Institute Inc filed Critical Massachusetts Institute of Technology Priority to PCT/US2015/032760 priority patent/WO2015184016A2/en Priority to US15/313,863 priority patent/US20170267997A1/en Publication of US20170267997A1 publication Critical patent/US20170267997A1/en Assigned to THE BROAD INSTITUTE, INC. Original Assignee Massachusetts Institute of Technology Broad Institute Inc Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Pending Application number US15/313,863 Inventor Robert Nicol Lauren WOODRUFF Tarjei Mikkelsen Christopher Voigt Current Assignee (The listed assignees may be inaccurate. Google Patents US20170267997A1 - High-throughput assembly of genetic elements US20170267997A1 - High-throughput assembly of genetic elements
0 kommentar(er)
